Meaning
An oligonucleotide that is complementary to the minus strand of an M13 sequencing vector (Sanger sequencing) and allows to sequence this strand from its 50 terminus (5' -> 3'). Usually, the plus strand is sequenced first starting at its 30 end (3' -> 5') using a normal sequencing primer. Minus strand sequencing serves to confirm the base sequence determined by plus strand sequencing (https://onlinelibrary.w...)
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