Adsorption chromatography (column, TLC, Chromatotron) of the petroleum ether extract from the roots of Lonchocarpus subglaucescens (Benth) - Leguminosae, was used to isolate twenty two flavonoids: 6",6"- dimethyl - chromene 2",3":7,8 flavone (1,9); 3 ',4 '-methylenodioxy-6",6"-dimethyl - chromene 2",3":7,8 flavone (1,0); (2,S) - 3',4'-methylenodioxy- 6",6" - dimethylchromene 2",3":7,8 flavanone (1,1); (2.S) - 3',4'- dimethoxy-6", 6"-dimethylchromene 2",3":7,8 flavanone (1,2); 6-methoxy- 6",6"- dimethylchromene 2",3":7,8 flavanone (1,3); (2,S) - 5,6-dimethoxy- 2",3":7,8 furanoflavanone (1,4); (2R,3R) - 3,5,6-trimethoxy-2",3":7,8 furanoflavanonol (1,5); (2,4-cis)-4,5,6-trimethoxy-2",3":7,8 furanoflavan (1,3); (2,4-cis) - 3',4'-methylenodioxy-4,5,8-trimethoxy-2",3":7,8 furanoflavan (1,4); (2,4-trans, 3,4-cis) - 3,4,5,6-tetramethoxy-2",3":7,8 furanoflavan (1,0); (2,4-trans 3,4-cis) - 3,4,5,8-tetramethoxy-2",3":7,6 furanoflavan (1,9); (2,4-trans, 3,4-cis) - 3',4'-methylenodioxy-3,4,5,6- tetramethoxy-2",3":7,8 furanoflavan (1,2); (2,4-trans, 3,4-cis) - 3',4'- methylenodioxy-3,4,5,8-tetramethoxy-2",3":7,6 furanoflavan (1,1); 3,4- dimethoxy-2'-hydroxy-6",6"-dimethylchromene 6",5,4',3' chalcone (1,6); 3,4-methylenodioxy - 2' - hydroxy - 3',6' - dimethoxy - 2",3":4',5' furanchalcone (1,7); 3,4-methylenodioxy - 2' - methoxy -2",3":4'~3' dihydrochalcone (1,8); 2'-methoxy-6",6"-dimethylchromene 6",5":4',3' b -hydroxychalcone (1,5); 3,4-methylenodioxy - 2' - methoxy - 6",6"- dimethylchromene 6",5":4 ',3' b-hydroxychalcone (1,6); (E)-3,4- methylenodioxy - 2'- methoxy - 6",6"-dimethylchromene 6",5":4',3' 9- methoxychalcone (1,7); (Z)-3,4-methylenodioxy- 2'- methoxy-6",6"- dimethylchromene 6",5":4',3' 9-methoxychalcone (1,8); (6ab,12ab)-12a-