For DNA extraction, the skin sample was digested with 1.0 mL of 1X digestion buffer [0.1 M Tris pH 8.0, 0.1 M EDTA, 1% SDS, 20 mg / ml proteinase K in PK buffer] at 55ºC, stirred overnight.
Para a extração do DNA, a amostra de pele foi digerida com 1,0 mL de buffer de digestão 1x [0,1M tris pH 8,0; 0,1M EDTA; 1% SDS; 20 mg/mL de proteinase K PK], a 55ºC, com agitação, overnight.
The viral DNA in the paraffin bloc samples was extracted by means of an enzymatic digestion using 200ml of digestion buffer TrisHCL 10mM, KCl 50mM, MgCl2 2.5mM, 0.5% Tween 20.
O DNA viral nas amostras de blocos de parafina foi extraído através de uma digestão enzimática, usando 200ml de um tampão de digestão TrisHCL 10mM, KCl 50mM, MgCl2 2.5mM, 0.5% Tween 20.